Description
Product Overview
The MACHEREY NAGEL Chromabond C18 ec SPE Polypropylene Column is engineered to meet the demanding requirements of modern liquid chromatography workflows. With a generous 6 ml column volume and a precisely measured 500 mg of high‑purity C18 bonded adsorbent, this column offers a balanced combination of capacity and efficiency that supports a wide range of analytical applications. The column is constructed from chemically resistant polypropylene, a material chosen for its durability, low extractable profile, and compatibility with a broad spectrum of solvents, including aggressive organic mixtures. This robust housing protects the stationary phase from mechanical stress while maintaining a consistent internal diameter, which is critical for reproducible flow rates and peak shapes.
Key specifications include a column length of 150 mm and an internal diameter of 4.6 mm, dimensions that are standard for most HPLC systems, ensuring seamless integration without the need for additional hardware modifications. The column dimensions are complemented by a compact footprint, with package dimensions of 5.87 × 5.67 × 4.37 inches and a lightweight design of 7.41 ounces, facilitating easy storage and handling in busy laboratory environments. The product model number 730014 uniquely identifies this configuration, allowing for precise inventory management and rapid ordering when replacements are needed.
Manufactured by MACHEREY NAGEL, a globally recognized leader in sample preparation technologies, the Chromabond series benefits from decades of expertise in sorbent chemistry and column engineering. The C18 ec (end‑capped) stationary phase provides a hydrophobic surface that minimizes secondary interactions, delivering sharp, symmetrical peaks and high recovery rates for a variety of analytes, from small molecules to larger biomolecules. The end‑capped design also reduces tailing caused by residual silanol groups, enhancing method robustness and reproducibility across multiple runs.
Usage
This column is ideally suited for a broad spectrum of analytical scenarios, including pharmaceutical impurity profiling, environmental monitoring, food safety testing, and clinical research. Its versatility stems from the C18 bonded phase, which efficiently retains non‑polar to moderately polar compounds, making it a go‑to choice for reverse‑phase separations. Laboratories that require high throughput can leverage the column’s 6 ml volume to process larger sample loads without compromising resolution, thereby reducing the number of injections needed for comprehensive analyses.
In practice, the Chromabond C18 ec SPE column excels in sample clean‑up steps prior to HPLC analysis. By loading complex matrices onto the column, analysts can selectively retain target analytes while washing away interfering substances, resulting in cleaner extracts and improved detector response. The column’s polypropylene construction also tolerates a wide pH range (typically 2–10), allowing users to employ diverse mobile phase conditions without risking degradation of the column hardware.
For routine quality‑control laboratories, the column offers a reliable platform for method validation, supporting parameters such as linearity, limit of detection, and precision. Its consistent performance across batches ensures that method transfer between instruments or sites can be achieved with minimal re‑optimization, saving valuable time and resources. The column’s compatibility with both conventional HPLC and ultra‑high‑performance liquid chromatography (UHPLC) systems further expands its applicability, providing flexibility as laboratories upgrade their instrumentation.
Why Choose Us
Choosing the MACHEREY NAGEL Chromabond C18 ec SPE Polypropylene Column means partnering with a brand that prioritizes quality, reliability, and customer support. Each column undergoes rigorous quality control testing, including performance verification under standardized conditions, to guarantee that the advertised specifications are met or exceeded. The manufacturing process incorporates state‑of‑the‑art bonding techniques that ensure uniform coverage of the C18 ligand, resulting in reproducible retention characteristics across the entire column length.
Our commitment to sustainability is reflected in the use of recyclable polypropylene and environmentally responsible production practices. By selecting this column, laboratories not only benefit from superior analytical performance but also contribute to greener laboratory operations. Additionally, MACHEREY NAGEL provides comprehensive technical documentation, including detailed method development guides and troubleshooting resources, empowering users to maximize the column’s potential.
Customer service is a cornerstone of our offering. Our dedicated support team is available to assist with method development, performance optimization, and any technical inquiries that may arise during the product lifecycle. Whether you are implementing a new analytical method or seeking to improve an existing workflow, our experts are ready to provide tailored recommendations and prompt resolutions.
Key Features
- Robust polypropylene housing ensures chemical resistance and long‑term durability
- 6 ml volume and 500 mg C18 ec adsorbent provide high capacity without sacrificing resolution
- End‑capped C18 phase minimizes peak tailing and improves reproducibility for diverse analytes
- Compatible with standard HPLC and UHPLC systems for flexible laboratory integration
- Dedicated technical support and comprehensive documentation for seamless method development
FAQ
What types of samples are best suited for this SPE column?
The Chromabond C18 ec SPE column is ideal for extracting non‑polar to moderately polar compounds from complex matrices such as plasma, urine, environmental water samples, food extracts, and pharmaceutical formulations. It effectively removes interfering substances while retaining target analytes for subsequent HPLC analysis.
Can the column be used with both HPLC and UHPLC systems?
Yes, the column’s dimensions and particle size are compatible with conventional HPLC as well as ultra‑high‑performance liquid chromatography platforms. Users can benefit from faster analysis times on UHPLC systems while maintaining the same stationary phase chemistry.
How does the end‑capped C18 phase improve chromatographic performance?
End‑capping reduces residual silanol activity on the silica surface, which can cause secondary interactions and peak tailing. By minimizing these interactions, the end‑capped C18 phase delivers sharper peaks, higher recovery, and more consistent retention times across multiple runs.
What is the recommended storage condition for the column?
Store the column in a cool, dry place away from direct sunlight. It is advisable to keep the column sealed in its original packaging until use to protect the stationary phase from moisture and contaminants.
Is there a warranty or guarantee for this product?
MACHEREY NAGEL offers a standard warranty that covers manufacturing defects for a period of 12 months from the date of purchase. Should any issues arise, customers can contact our support team for replacement or troubleshooting assistance.
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