Description
Product Overview
The MACHEREY-NAGEL Nucleosil 100-7 C18 HPLC column is engineered for high performance in analytical chromatography. With a precise 125 mm length and a standard 4.6 mm internal diameter, this column delivers reproducible results across a broad spectrum of applications. The stationary phase consists of end‑capped C18 bonded silica particles, each 5 µm in size, providing a uniform pore structure of 100 Å. This combination ensures excellent peak shape, low backpressure, and minimal silanol activity, making it suitable for both routine and demanding analytical tasks. The column is compatible with a wide range of mobile phases, including aqueous, organic, and mixed solvents, and can be used under isocratic or gradient conditions. Its robust construction and high‑quality manufacturing standards guarantee long‑term stability and consistent performance, even under intensive usage.
Usage
Designed for laboratory environments, the Nucleosil 100-7 C18 column excels in pharmaceutical analysis, environmental testing, food safety, and academic research. It is ideal for the separation of small molecules, peptides, and moderately sized biomolecules, providing reliable retention and resolution for complex mixtures. The column’s hydrophobic interaction mechanism enables effective separation of compounds based on van der Waals forces, while the slight residual silanol activity offers additional selectivity when needed. Users can integrate this column into existing HPLC systems without the need for specialized hardware, as it conforms to standard dimensions and fittings. Routine maintenance involves simple flushing with appropriate solvents, and the column can be stored in a sealed container to preserve its performance between runs.
Why Choose Us
Choosing the MACHEREY-NAGEL Nucleosil 100-7 C18 column means selecting a product backed by decades of expertise in chromatography media development. Each column undergoes rigorous quality control, including particle size distribution analysis, pore size verification, and end‑capping efficiency testing, ensuring that you receive a consistent and reliable tool for your analytical needs. The brand’s commitment to innovation is reflected in the use of spherical silica particles, which were among the first of their kind to be employed in HPLC, delivering superior packing efficiency and reduced band broadening. Additionally, our dedicated technical support team provides guidance on method development, troubleshooting, and optimization, helping you achieve the best possible results with minimal downtime.
Key Features
- End‑capped C18 phase delivers low silanol activity for clean baselines.
- 5 µm spherical silica particles provide high efficiency and low backpressure.
- 100 Å pore size accommodates a wide range of molecular sizes.
- Standard 125 mm × 4.6 mm dimensions fit most HPLC systems without modification.
- Comprehensive technical support ensures rapid assistance and method optimization.
FAQ
What types of samples are best suited for this column?
The column is ideal for small organic molecules, pharmaceuticals, pesticides, and moderate‑size peptides. Its hydrophobic C18 phase provides strong retention for non‑polar compounds while still allowing adequate separation of more polar analytes.
How long can the column be used before replacement is needed?
With proper maintenance—regular flushing with compatible solvents and avoiding extreme pH conditions—the column can reliably perform thousands of injections, often exceeding the typical service life of comparable analytical columns.
Can this column be used for gradient elution?
Yes, the Nucleosil 100-7 C18 column is fully compatible with gradient elution methods. Its stable packing and low bleed characteristics make it suitable for complex gradient programs that require precise control over retention times.
Is the column compatible with high‑temperature operation?
The column is rated for temperatures up to 60 °C, allowing you to increase mobile phase temperature for improved mass transfer and reduced viscosity when needed.
What support is available if I encounter performance issues?
Our expert technical support team can assist with troubleshooting, method development, and optimization. You can contact us via phone, email, or our online portal for rapid assistance.
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