MACHEREY NAGEL Nucleosil 100-10 C18 HPLC Column 250mm 4.6mm ID

Description

Product Overview

The MACHEREY NAGEL Nucleosil 100-10 C18 HPLC column is designed for high‑performance analytical chromatography. It features a 250 mm length and a 4.6 mm internal diameter, providing a balance of resolution and back‑pressure suitable for most laboratory HPLC systems. The column is packed with 5 micron spherical silica particles that have a uniform 100 Angstrom pore size, delivering consistent flow characteristics and excellent peak shape. Endcapped chemistry reduces residual silanol activity, minimizing tailing for basic compounds while maintaining strong hydrophobic retention for a wide range of analytes. The column is compatible with standard USP L1 specifications, ensuring reliable performance across diverse method development projects. Its robust construction and precise tolerances make it a dependable choice for routine analyses in pharmaceutical, environmental, food, and biotech laboratories.

Usage

This column excels in applications that require reliable reversed‑phase separations, such as the analysis of small molecules, peptides, and metabolites. It is well‑suited for method development in drug discovery, quality control, and regulatory testing where reproducibility and robustness are critical. The column can be operated under a wide range of mobile phase compositions, from aqueous buffers to high‑organic solvent gradients, making it flexible for both polar and non‑polar analytes. Typical flow rates range from 0.5 to 1.5 mL min⁻¹, and the column tolerates temperatures up to 60 °C, allowing users to fine‑tune selectivity and efficiency. Its durability supports thousands of injections, reducing consumable costs over time. Ideal users include analytical chemists, quality assurance specialists, and research scientists who need a dependable column for high‑throughput screening or detailed impurity profiling.

Why Choose Us

Choosing the MACHEREY NAGEL Nucleosil C18 column means selecting a product backed by decades of chromatographic expertise and rigorous quality control. Each column undergoes strict testing for dimensional accuracy, particle uniformity, and chemical stability, ensuring that performance matches the manufacturer’s specifications. The endcapped surface chemistry provides low background noise and high reproducibility, which translates into consistent results across multiple runs and laboratories. Our supply chain guarantees that the column arrives in optimal condition, with protective packaging that prevents damage during transit. In addition, we offer technical support from experienced application scientists who can assist with method optimization, troubleshooting, and validation documentation. This comprehensive service package helps customers achieve reliable data faster, minimizing downtime and accelerating project timelines.

Key Features

• Reliable hydrophobic retention for a broad range of analytes, improving method robustness.
• Uniform 5 micron particles and 100 Angstrom pores deliver sharp peaks and consistent flow.
• Endcapped silica reduces silanol interactions, minimizing peak tailing for basic compounds.
• Compatible with standard HPLC systems and USP L1 specifications for easy integration.
• Dedicated technical support and after‑sales service to help you optimize performance.

FAQ

What types of samples are best suited for this column?

The column is ideal for reversed‑phase separations of small molecules, peptides, metabolites, and environmental contaminants. Its balanced chemistry works well with both polar and non‑polar compounds, making it versatile for pharmaceutical, food safety, and environmental testing.

Can the column be used at elevated temperatures?

Yes, the column is rated for operation up to 60 °C. Higher temperatures can improve mass transfer and reduce analysis time, but users should verify method stability and detector compatibility when increasing temperature.

How many injections can the column handle before performance degrades?

Under typical analytical conditions, the column can sustain several thousand injections while maintaining resolution and efficiency. Proper maintenance, such as regular flushing with appropriate solvents, extends column life.

Is the column compatible with gradient elution methods?

Absolutely. The column performs well with gradient programs ranging from low to high organic solvent percentages. Its stable packing and endcapped surface ensure consistent retention and peak shape throughout the gradient.

What support is available if I encounter issues?

Our technical support team provides guidance on method development, troubleshooting, and validation. Customers can contact us via phone, email, or online portal for rapid assistance and detailed application notes.