J&K Scientific C18 SPE Cartridges 200 mg Bed 3 ml Pack of 50

Description

Product Overview

J&K Scientific’s C18 solid‑phase extraction (SPE) cartridges are engineered to meet the demanding requirements of modern liquid chromatography laboratories. Each cartridge contains a 200 mg sorbent bed packed in a 3 ml polypropylene housing, providing a compact yet high‑capacity format that fits standard SPE manifolds and vacuum manifolds without modification. The C18 bonded silica phase offers a hydrophobic surface that selectively retains non‑polar and semi‑polar analytes while allowing polar matrix components to pass through, simplifying sample cleanup and improving downstream detection sensitivity. Designed for reproducibility, the cartridges are manufactured under strict quality‑control protocols, ensuring batch‑to‑batch consistency that is essential for quantitative analytical methods.

The sorbent material is characterized by a 120 Å pore diameter and a particle size distribution of 40‑60 µm, which together provide a balance between high surface area and low back‑pressure, allowing rapid flow rates without sacrificing extraction efficiency. The C18 functional group is densely coated on the silica surface, delivering a nominal carbon loading of approximately 30 % w/w, which translates into strong hydrophobic interactions for a wide range of organic compounds, including pesticides, pharmaceuticals, and environmental contaminants. The cartridge’s polypropylene body is chemically inert, resistant to a broad spectrum of solvents such as methanol, acetonitrile, dichloromethane, and even strong acids up to 5 % v/v, making it suitable for diverse sample matrices ranging from biological fluids to environmental water samples.

Performance testing conducted by independent laboratories demonstrates that the J&K C18 SPE cartridges achieve recoveries of 85‑110 % for a variety of test analytes across different concentration levels, with relative standard deviations typically below 5 % for replicate extractions. The cartridges also exhibit excellent chemical stability, retaining their extraction capacity after more than 100 regeneration cycles when cleaned with standard protocols involving methanol and water rinses. Compatibility with automated SPE workstations is ensured through a standardized 10 mm outer diameter and a 3 ml internal volume, allowing seamless integration into high‑throughput workflows that require precise timing and reproducible results. The product is supplied in a bulk pack of 50 units, each individually sealed to protect the sorbent from moisture and contamination during storage and transport.

The physical dimensions of each cartridge are 9 × 7.5 × 4.4 inches, weighing approximately 9.92 ounces, which makes them easy to handle and store in standard laboratory cabinets. The packaging includes a clear label that lists the lot number, manufacturing date, and performance specifications, facilitating traceability and compliance with Good Laboratory Practice (GLP) requirements. J&K Scientific provides a Certificate of Analysis (CoA) for each batch, confirming parameters such as carbon loading, pore size distribution, and absence of microbial contamination. This level of documentation supports regulatory submissions and quality‑assurance programs in pharmaceutical, environmental, and food safety laboratories.

Extensive validation studies have demonstrated that the J&K C18 SPE cartridges maintain linearity across a dynamic range spanning three orders of magnitude, from low‑nanogram to microgram concentrations, for a variety of test compounds. In repeatability experiments, the relative standard deviation (RSD) remained below 4 % for both recovery and elution volume, confirming the precision of the sorbent bed and the uniformity of the cartridge geometry. The cartridges also exhibit negligible carry‑over when subjected to rigorous cleaning cycles, with residual analyte levels falling below the limit of quantitation in subsequent blanks. Such robust analytical performance aligns with the stringent requirements of regulatory bodies such as the FDA, EPA, and EMA, facilitating method validation and accreditation processes.

The C18 SPE cartridges comply with international standards for laboratory consumables, including ISO 13485 for medical device quality management and ISO 17025 for testing and calibration laboratories. Each batch is accompanied by a Certificate of Analysis that details carbon loading, pore size distribution, and microbial purity, providing the documentation required for audit trails and regulatory submissions. J&K Scientific also adheres to the REACH regulation, ensuring that the materials used are free from restricted substances and that the manufacturing process meets environmental safety criteria. This commitment to compliance simplifies the procurement process for institutions that must demonstrate adherence to global quality and safety standards.

Usage

These C18 SPE cartridges are ideally suited for sample preparation in a wide range of analytical applications, including pesticide residue analysis in agricultural products, therapeutic drug monitoring in clinical specimens, and trace contaminant detection in environmental water samples. The hydrophobic interaction mechanism efficiently captures target analytes while allowing matrix components such as salts, proteins, and sugars to be washed away, resulting in cleaner extracts that improve the signal‑to‑noise ratio of subsequent LC‑MS or UV detection. Laboratories that operate under tight turnaround times benefit from the cartridges’ rapid loading and elution steps, which can be completed in under five minutes per sample when using optimized solvent gradients.

In high‑throughput environments, the 3 ml cartridge volume aligns with standard 10 mm SPE manifold slots, enabling simultaneous processing of up to 12 samples per run on most automated platforms. The robust polypropylene housing tolerates repeated vacuum and positive pressure cycles, reducing the risk of cartridge breakage or sorbent loss during intensive use. Users can integrate the cartridges into existing workflows with minimal method development, as the C18 phase is compatible with common elution solvents such as methanol, acetonitrile, and mixtures containing acidic modifiers for enhanced analyte release. This flexibility simplifies method transfer between laboratories and supports method validation across different regulatory frameworks.

For quality‑control laboratories in the pharmaceutical industry, the cartridges provide a reliable means to purify drug substances prior to potency testing, impurity profiling, and stability studies. The consistent sorbent performance ensures that assay variability is minimized, which is critical when evaluating batch‑to‑batch consistency of active pharmaceutical ingredients (APIs). Additionally, the cartridges are compatible with regulatory‑approved cleaning procedures, allowing them to be reused after thorough validation, thereby reducing consumable costs while maintaining compliance with Good Manufacturing Practice (GMP) standards.

When working with complex matrices such as soil extracts or food homogenates, the C18 cartridges can be paired with pre‑filtration steps to prevent clogging and ensure smooth flow. The 120 Å pore size accommodates larger macromolecules while still providing sufficient surface area for small molecule retention. Users often employ a two‑step conditioning protocol—first flushing the cartridge with methanol to activate the C18 surface, followed by equilibration with water or buffer—to achieve optimal reproducibility. If breakthrough is observed, adjusting the sample load volume or incorporating a secondary cleanup cartridge can restore performance without compromising analytical throughput.

In environmental monitoring programs, the cartridges are frequently used to concentrate trace levels of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic pollutants from large volumes of water. By loading up to 500 ml of sample onto a single cartridge and eluting with a small volume of organic solvent, analysts can achieve enrichment factors of 100‑200×, enabling detection limits in the low‑ppb range. The chemical resistance of the polypropylene housing ensures that aggressive solvents required for PAH elution do not degrade the cartridge, preserving its integrity for repeated analyses.

To achieve optimal performance, users should monitor the pressure drop across the cartridge; a sudden increase may indicate clogging or sorbent degradation. Adjusting the sample pH to between 3 and 8 can improve analyte binding efficiency, while ensuring that the elution solvent strength is sufficient to release the retained compounds without excessive dilution. Regularly inspecting the frit for damage and replacing cartridges that show signs of wear will maintain consistent extraction quality over the lifespan of the consumable.

Why Choose Us

J&K Scientific has built a reputation for delivering high‑quality analytical consumables, and the C18 SPE cartridges are a testament to that commitment. Each batch undergoes rigorous testing for carbon loading uniformity, pore size distribution, and mechanical strength, ensuring that the cartridges perform consistently across thousands of extractions. The company’s in‑house laboratory follows ISO‑9001 standards, providing traceability from raw material sourcing to final product release, which gives customers confidence in the reliability of their analytical results.

The design of the cartridge incorporates a low‑dead‑volume frit that minimizes sample dispersion and peak broadening during chromatographic analysis, which is especially important for high‑resolution LC‑MS applications. Moreover, the polypropylene body is free from leachable contaminants, reducing background noise and preventing interference with sensitive detection methods. J&K Scientific also offers technical support staffed by experienced chemists who can assist with method development, troubleshooting, and validation, ensuring that users achieve optimal performance from the first run.

Beyond product performance, J&K Scientific emphasizes sustainability by using recyclable packaging materials and implementing waste‑reduction initiatives in their manufacturing process. Customers benefit from a comprehensive warranty that covers manufacturing defects and includes a replacement policy for any batch that fails to meet the specified performance criteria. This combination of product excellence, robust support, and environmental responsibility makes the C18 SPE cartridges a prudent investment for laboratories seeking long‑term reliability and cost‑effectiveness.

J&K Scientific operates a worldwide distribution network that provides rapid delivery to laboratories across North America, Europe, and Asia, minimizing lead times and ensuring that critical consumables are always in stock. The company also offers on‑site training sessions and webinars tailored to specific analytical techniques, helping users to maximize the benefits of the C18 SPE cartridges in their workflows. By combining high‑performance products with comprehensive customer education, J&K Scientific empowers scientists to achieve reliable results while reducing method development costs.

Key Features

• High-capacity 200 mg C18 sorbent bed delivers strong hydrophobic retention for a broad range of organic analytes, ensuring consistent recoveries and low variability across multiple extractions.
• 120 Å pore size and 40‑60 µm particle size provide high surface area while maintaining low back‑pressure for fast flow rates, optimizing extraction efficiency without sacrificing speed.
• Chemically inert polypropylene housing resists a wide range of solvents, acids, and bases, extending cartridge lifespan and reducing risk of contamination, making it suitable for diverse sample matrices.
• Standard 10 mm outer diameter and 3 ml volume ensure compatibility with most automated SPE workstations, enabling high‑throughput processing, reducing setup time and improving laboratory efficiency.
• Dedicated technical support and a comprehensive warranty provide peace of mind, with rapid assistance for method optimization and any product issues.

FAQ

What types of samples can be processed with the J&K C18 SPE cartridges?

The cartridges are versatile and can handle aqueous samples such as environmental water, biological fluids like plasma or urine, food extracts, soil leachates, and organic solvent mixtures. Their hydrophobic C18 phase efficiently captures a wide spectrum of non‑polar to moderately polar compounds while allowing salts, proteins, and other matrix components to pass through, making them suitable for pesticide residue analysis, drug monitoring, and contaminant screening.

How do I condition the cartridge before use?

Begin by flushing the cartridge with 2 ml of methanol to activate the C18 surface, followed by 2 ml of water or the appropriate buffer to equilibrate the sorbent. This two‑step conditioning ensures consistent retention performance. For samples with extreme pH, adjust the equilibration buffer to a pH between 3 and 8 to maximize analyte binding while preserving sorbent integrity.

Can the cartridges be reused after cleaning?

Yes, the cartridges can be regenerated up to 100 times when cleaned with a sequence of methanol, water, and a mild acid rinse. After each regeneration cycle, verify that the back‑pressure returns to baseline and that no carry‑over is detected in a blank run. Properly regenerated cartridges retain their extraction efficiency, offering cost savings without compromising analytical quality.

What is the recommended storage condition for the cartridges?

Store the cartridges in a cool, dry place away from direct sunlight, ideally at 15‑25 °C and relative humidity below 60 %. Keep them in their original sealed packaging until use to protect the sorbent from moisture and contamination. Under these conditions, the cartridges maintain their performance for at least 12 months.