Hamilton 79889 HxSil C18 HPLC Column 2.1x50mm Stainless Steel 3µm

Description

Product Overview

The Hamilton 79889 HxSil C18 HPLC column is a purpose‑built solution for laboratories that demand consistent, high‑performance reversed‑phase separations. Constructed with a robust stainless‑steel housing, the column protects the packed silica bed from mechanical stress, particulate intrusion, and the aggressive action of strongly adsorptive compounds. Inside, a 3 µm silica packing with a uniform 100 Å pore size delivers sharp, reproducible peaks across a broad range of analytes, from small organic molecules to moderately sized peptides. The column dimensions—2.1 mm internal diameter and 50 mm length—provide a compact footprint while maintaining sufficient column volume for efficient separation, making it an excellent choice for high‑throughput workflows and limited sample volumes. Engineered to operate effectively within a mobile‑phase pH window of 2.0 to 7.5, the HxSil C18 chemistry offers flexibility for a variety of buffer systems, including aqueous, mixed‑organic, and buffered environments. The product is supplied by Thomas Scientific, a trusted distributor in the scientific community, and meets rigorous quality standards to ensure reliable performance batch after batch. Whether you are developing new pharmaceutical compounds, performing environmental contaminant analysis, or conducting routine quality control, this column delivers the precision and durability required for demanding analytical applications.

Usage

Designed for versatility, the Hamilton 79889 HxSil C18 column excels in a range of laboratory settings. In pharmaceutical research, it supports method development for active pharmaceutical ingredient (API) purification, impurity profiling, and stability testing, handling both acidic and near‑neutral mobile phases with ease. Biotechnology laboratories benefit from its ability to separate complex mixtures of metabolites, peptides, and small proteins, providing clear resolution even when dealing with challenging sample matrices. Environmental testing facilities rely on the column’s robustness to analyze water and soil extracts, detecting trace levels of pesticides, herbicides, and industrial contaminants. The 2.1 mm × 50 mm format is compatible with standard UHPLC and HPLC systems, allowing seamless integration into existing workflows without the need for extensive hardware modifications. Its stainless‑steel construction also tolerates higher back‑pressures, enabling the use of elevated flow rates for rapid analysis while preserving column integrity. Users can employ the column in gradient elution methods, isocratic runs, or mixed‑mode separations, taking advantage of the C18 stationary phase’s well‑known hydrophobic interactions. The column’s broad pH tolerance simplifies method transfer between different laboratories, ensuring consistent results across diverse analytical platforms. Overall, the Hamilton 79889 HxSil C18 column provides a reliable, high‑performance tool for scientists seeking accurate, reproducible data in pharmaceutical, biotech, and environmental applications.

Why Choose Us

Choosing the Hamilton 79889 HxSil C18 column means partnering with a brand renowned for scientific excellence and a commitment to quality that spans decades. Each column undergoes stringent manufacturing controls, including precise particle size distribution verification and pore size consistency checks, ensuring that every unit meets the exacting standards required for high‑resolution chromatography. The stainless‑steel hardware not only extends column lifespan but also minimizes the risk of metal‑catalyzed degradation of sensitive analytes, a critical factor for labs handling reactive or labile compounds. Our distribution network, anchored by Thomas Scientific, provides reliable logistics, rapid order fulfillment, and responsive technical support, so you can focus on your research rather than supply chain concerns. In addition, we offer comprehensive documentation, including detailed specifications, recommended operating conditions, and troubleshooting guides, empowering users to optimize performance from day one. Our after‑sales service includes expert advice on method development, column regeneration, and compatibility assessments, ensuring that you extract maximum value from your investment. By selecting this Hamilton column, you gain access to a product that combines proven chemistry, durable construction, and a support ecosystem designed to accelerate scientific discovery and maintain analytical integrity.

Key Features

• Robust stainless‑steel housing protects the silica bed from particulates and harsh chemicals, extending column life.
• 3 µm silica particles with 100 Å pores deliver high efficiency and sharp peak shapes for a wide range of analytes.
• Compact 2.1 mm × 50 mm dimensions enable fast analysis while conserving solvent and sample volume.
• Broad pH compatibility (2.0–7.5) allows flexible mobile‑phase selection for diverse method development needs.
• Dedicated technical support and detailed documentation ensure smooth implementation and ongoing performance optimization.

FAQ

What types of samples are best suited for this column?

The Hamilton 79889 HxSil C18 column is ideal for small‑molecule pharmaceuticals, peptide fragments, metabolites, and environmental contaminants. Its C18 chemistry provides strong hydrophobic interactions, making it effective for separating compounds with varying polarity in both aqueous and mixed‑organic mobile phases.

Can this column be used with high‑pressure UHPLC systems?

Yes. The stainless‑steel hardware is designed to withstand elevated back‑pressures typical of UHPLC operation. Users can run the column at higher flow rates to achieve faster separations without compromising column integrity or performance.

How do I maintain column performance over time?

Regular column care includes flushing with appropriate solvents after each run, avoiding prolonged exposure to extreme pH or strong solvents, and storing the column in a sealed container with a protective solvent when not in use. Following the manufacturer’s recommended cleaning protocols will help preserve efficiency and extend service life.

Is the column compatible with gradient elution methods?

Absolutely. The HxSil C18 packing provides consistent retention behavior across gradient conditions, allowing seamless method development from isocratic to complex gradient programs. Its stable chemistry ensures reproducible results even when the mobile‑phase composition changes rapidly.

What support is available if I encounter issues?

Our technical support team, accessible through Thomas Scientific, offers expert guidance on method optimization, troubleshooting, and column regeneration. Detailed user manuals, application notes, and direct contact with experienced scientists are provided to help resolve any challenges quickly.